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Waste Management & Research, Vol. 13, No. 2, 137-147 (1995)
DOI: 10.1177/0734242X9501300204

Measurement of Microbial Biomass and Activity in Landfill Soils

J.E. Bogner

Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, U.S.A.

R.M. Miller

Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, U.S.A.

K. Spokas

Argonne National Laboratory, 9700 South Cass Avenue, Argonne, Illinois 60439, U.S.A.

Two complementary techniques, which have been widely used to provide a general measure of microbial biomass or microbial activity in natural soils, were evaluated for their applicability to soils from the Mallard North and Mallard Lake Landfills, DuPage County, Illinois, U.S.A. Included were: (1) a potassium sulphate extraction technique with quantification of organic carbon for measurement of microbial biomass; and (2) an arginine ammonification technique for microbial activity. Four profiles consisting of replaced soils were sampled for this study; units included topsoil (mixed mollisol A and B horizons), compacted clay cover (local calcareous Wisconsinan age glacial till), and mixed soil/refuse samples. Internally consistent results across the four profiles and good correlations with other independent indicators of microbial activity (moisture, organic matter content, nitrogen, and phosphorus) suggest that, even though these techniques were developed mainly for natural mineral soils, they are also applicable to disturbed landfill soils.

Key Words: Landfill microbiology • landfill soils • arginine ammonification • microbial biomass.


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