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Kinetics of CH4 oxidation in mixed culture

Department of Civil and Environmental Engineering, Miyazaki University, 1-1 Gakuenn Kibanadai Nishi, Miyazaki 889-2192, Japan, dote{at}civil.miyazaki-u.ac.jp

Microbial cultures used for methane oxidation experiments were prepared by enriching under a 20% CH₄ atmosphere either leachate extracts from two different landfill sites or a digested sewage sludge. No microbes were axenically isolated, therefore the cultures were mixtures of CH₄ oxidising and other aerobic microorganisms. CH₄ oxidation experiments were carried out with a batch reactor, whose change in gas volume could be measured. A mixture of air and methane with CH₄ concentrations ranging from 5 to 20% was introduced into the reactor. Methane oxidation rate was expressed as a Monod equation for CH₄ and a first order reaction for O₂. Maximum oxidation rate (V_max) resulted 2.1x10^-13 to 3.4x10^-11 hr^-1cell^-1, and half saturation constant (K_M) was 5.1x10^-5 to 6.5x10^-4 mol L^-1. The molar ratio of consumed O₂ to oxidised CH₄ was 1.5-1.8

Key Words: Methane oxidation • kinetics • mixed culture • batch reactor • leachate • digested sewage sludge • wmr 578-6

Waste Management & Research, Vol. 20, No. 6, 494-500 (2002)
DOI: 10.1177/0734242X0202000603


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