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Synergism effects of phenol-degrading yeast and ammonia-oxidizing bacteria for nitrification in coke wastewater of Esfahan Steel Company

Department of Biology, Faculty of Science, Isfahan University, Isfahan, Iran, ghanavatih{at}yahoo.com

Giti Emtiazi

Department of Biology, Faculty of Science, Isfahan University, Isfahan, Iran

Mehdi Hassanshahian

Department of Biology, Faculty of Science, Isfahan University, Isfahan, Iran

Ammonia-oxidizing bacteria and phenol-degrading yeast were isolated in order to study the synergism effects of phenol-degrading yeast and ammonia-oxidizing bacteria for enhancing the nitrification in coke wastewater from the Isfahan Steel Company. The influent and effluent samples with 600—1200 mg L^{— 1} ammonium and 550—2350 mg L^—1 phenol were collected aseptically in sterile flasks. The biodegradation of phenol and nitrification were studied with different treatments. The results showed that addition of Na₂CO₃ and autotrophic bacteria to wastewater increased the ammonium removal by 100%. Furthermore, the synergism effects of phenol-degrader yeast and autotrophic bacteria reduced the time for ammonium removal.

Key Words: Nitrification • ammonia-oxidizing bacteria • phenol-degrader yeast • coke wastewater • simultaneous removal of ammonia and phenol • wmr 1108—3

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