This Article Full Text (PDF) All Versions of this Article: 0734242X08090405v1 27/3/224    most recent References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Mokni-Tlili, S. Articles by Hassen, A. Search for Related Content PubMed PubMed Citation Articles by Mokni-Tlili, S. Articles by Hassen, A. Social Bookmarking                         What's this?

Study of the effects of urban organic residues on the distribution of culturable actinomycetes in a Tunisian agricultural soil

Laboratoire de Traitement et Recyclage des Eaux Usées, Centre de Recherches et Technologies des Eaux, Technopole de Borj Cedria, Soliman, Tunisia, mt_sonia{at}yahoo.fr

Leila Jaoua

Laboratoire de Traitement et Recyclage des Eaux Usées, Centre de Recherches et Technologies des Eaux, Technopole de Borj Cedria, Soliman, Tunisia

Fumio Murano

Laboratoire de Traitement et Recyclage des Eaux Usées, Centre de Recherches et Technologies des Eaux, Technopole de Borj Cedria, Soliman, Tunisia

Naceur Jedidi

Laboratoire de Traitement et Recyclage des Eaux Usées, Centre de Recherches et Technologies des Eaux, Technopole de Borj Cedria, Soliman, Tunisia

Abdennaceur Hassen

Laboratoire de Traitement et Recyclage des Eaux Usées, Centre de Recherches et Technologies des Eaux, Technopole de Borj Cedria, Soliman, Tunisia

The main objective of this investigation was to identify a collection of actinomycetes isolates and to study the influence of amendment [municipal solid waste compost (MSWC) and farmyard manure (FM)] on their distribution in agricultural soil. For this purpose, a phenotypic and molecular characterization of 226 isolates collected from soil (with and without amendment) and 55 isolates from MSWC and FM was developed. The phenotypic study showed that the majority of strains isolated belong to the genus Streptomyces. By using the 16S rDNA polymerase chain reaction—restriction fragment length polymorphism method (restriction digest using six enzymes AluI, HhaI, MspI, TaqI , RsaI and HaeIII), two clusters were found: Streptomyces, dominant genus and Amycolatopsis, followed by Nocardioides. This result agreed with phylogeny revealed by 16S rDNA sequencing. The number of these actinomycetes in soil increased with FM or MSWC application. The studied soil is a potential source for isolation of actinomycetes, especially Streptomyces, and the application of organic amendment to the soil appeared to have an impact on the diversity of actinomycetes. Amendment of the soil with MSWC and FM significantly increased the number of actinomycetes due to the contribution of bacteria originally contained in biowastes and/or by stimulation of the endogenous soil micro-organisms.

Key Words: Actinomycetes • Streptomyces • restriction fragment length polymorphism • soil • municipal solid waste compost • farmyard manure • wmr 1280—6

This version was published on May 1, 2009

Waste Management & Research, Vol. 27, No. 3, 224-232 (2009)
DOI: 10.1177/0734242X08090405


CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?